# working folder /var/www/jbrowse/Jay_Zhu # empty the folder docs/fna # empty the folder docs/gff # empty the folder data/names # empty the folder data/seq # Copy Genome Info to the fna and gff folders copy all fna to ../docs/fna copy all gff to ../docs/gff cp /var/ngs/bowtie_indexes/bowtie_indexes/NC_000964.fna /var/www/jbrowse/Mirjam_Boonstra/docs/fna/NC_000964.fna cp /var/ngs/bowtie_indexes/bowtie_indexes/SMALL_RNA_GFF_Bsubt168.gff /var/www/jbrowse/Mirjam_Boonstra/docs/gff/SMALL_RNA_GFF_Bsubt168.gff # change header to >NC_xxx if not done yet bin/prepare-refseqs.pl --fasta docs/fna/NC_000964.fna # add annotation bin/flatfile-to-json.pl --gff docs/gff/SMALL_RNA_GFF_Bsubt168.gff --trackLabel Original_Annotation --trackType CanvasFeatures # make feature searchable bin/prepare-refseqs.pl -gff docs/gff/SMALL_RNA_GFF_Bsubt168.gff # active the names bin/generate-names.pl # make link in the /data folder to the data folder ln -s /home/anne/PROJECTS/mirjam/2016_03 dataset01 ln -s /home/anne/PROJECTS/mirjam/2017_03 dataset2017_03 # make data/tracks.conf file # usefull tool is to convert a table direct to a tracks.conf file. # goto /data and run: /usr/molgentools/tools/jbrowse_table_2_tracks.pl -i tracks_conf.table > tracks.conf # Change default spacing between tracks open jbrowse.conf and add the line "view.trackPadding = 7"